microPublication Biology2578-9430Caltech Library10.17912/W2MW2DCommodity ValidationNew FindingRetractedPhenotype DataC. elegans
Mutations in KCNQ potassium channels cause pharyngeal pumping defects in
C. elegansClovisYoanne1§WebbAlexis1TurnerCarl 1RobertsBill1
NemaMetrix, Inc,. 44 W 7th Ave., Eugene, OR 97401 USA.
WilliamsDaniel
Correspondence to: Yoanne Clovis (
yoanne.clovis@nemametrix.com
)
Pumps were stimulated with 10mM 
5HT
 in M9 recorded as electropharyngeograms (EPGs) for 2 minutes in a NemaMetrix ScreenChip, and analyzed using NemAnalysis software (NemaMetrix). The null mutant strains 
kqt-1(aw3) and
 
kqt-3 (aw1) 
were kindly donated by Dr. Aguan Wei (Wei et al., 2002)
A) Pump frequency in 
kqt-1
(
aw3
) animals was significantly lower than in 
N2
s, while 
kqt-3
(
aw1
) worms showed an increase in pump frequency (*p<0.05; ***p<0.01; 1-tailed Mann-Whitney U-test; n = 21-23 worms in each strain).
B) Microfluidic EPG recordings show that pumping pattern in 
kqt-1
(
aw3
) mutants is arrhythmic, with frequent drops in frequency.
C) Overlay of first 50 pumps of recordings show that pump duration is higher in 
kqt-1
(
aw3
) and 
kqt-3
(
aw1
) animals than in 
N2
s. Pumps are showed aligned on E spikes, which occur when the pharynx is fully contracted.
D) Duration histogram illustrating the probability of occurrence of inter-pump interval (E to E duration) for each mutant strain. Histograms were binned to 4 ms width and normalized to reach an area underneath the curve equal to 1 (duration 100% likelihood to occur). In 
kqt-1
(
aw3
)
)
 animals, the time between two pumps is significantly increased compared to N2s (p < 0.01).
Reagents
Molecule: 
Serotonin
Control Strain: 
N2
Funding
NemaMetrix, LLC
WeiAYuanAFawcettGButlerADavisTXuSYSalkoffL20021015Efficient isolation of targeted Caenorhabditis elegans deletion strains using highly thermostable restriction endonucleases and PCR.Nucleic Acids Res30200305-1048e110e11010.1093/nar/gnf10912384612